AMPK (A1/B2/G1), Active(P50-10H)

AMPK (A1/B2/G1), Active(P50-10H)

  • $395.00


Description :Recombinant full-length human AMPK (combination of A1/B2/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tags.

Species :Human

Tag :HIS tags

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_006251

Genbank Number 2 :NM_005399

Genbank Number 3 :NM_002733

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~68kDa (A1), ~36kDa (B2), ~40kDa (G1)

Gene Aliases :Subunit A1: PRKAA1, MGC33776, MGC57364 Subunit B2: PRKAB2, MGC61468 Subunit G1: PRKAG1, AMPKG, MGC8666

Scientific Background :AMPK (A1/B2/G1) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress (1). AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The β subunit may be a positive regulator of AMPK activity and is highly expressed in skeletal muscle (2).

References :
1. Viollet, B. et al: Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem. Soc. Trans. 2003; 31; 216–219.

2. Thornton, C. et al: Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle. J. Biol. Chem. 273: 12443-12450, 1998.

Formulation :50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 2mM DTT, 25% glycerol.

Product Sheets (By Lot #) :




Research Areas :Cancer, Metabolic Disorder, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases, Cancer, Metabolic Disorder, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases