Due to holidays, SignalChem's last days of shipping will be as follow: International Dec 14, 2018, US Dec 19, 2018. The shipments will resume on International Jan 4, 2019, and US Jan 7, 2019, as usual.
AMPK (A1/B1/G3), Active(P56-10H)

AMPK (A1/B1/G3), Active(P56-10H)

  • $435.00


FOR BULK ORDER REQUESTS PLEASE CONTACT US

Description :Recombinant full-length human AMPK (combination of A1/B1/G3 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tags.

Species :Human

Tag :HIS tags

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_006251

Genbank Number 2 :NM_006253

Genbank Number 3 :NM_017431

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~68kDa (A1), ~38kDa (B1), ~51kDa (G3)

Gene Aliases :Subunit A1: PRKAA1, MGC33776, MGC57364 Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G3: PRKAG3

Scientific Background :AMPK (A1/B1/G3) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress (1). AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The γ subunit is dominantly expressed in skeletal muscle where it may play a key role in the regulation of energy metabolism (2).

References :
1. Viollet, B. et al: Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models. Biochem. Soc. Trans. 2003; 31; 216–219.

2. Cheung, P. C. F. et al: Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding. Biochem. J. 346: 659-669, 2000.

Product Sheets (By Lot #) :

Q114-1.pdf

E299-2.pdf

Research Areas :Cancer, Metabolic Disorder, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases, Cancer, Metabolic Disorder, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases