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AMPK (A1/B1/G2), Active(P55-10H)

AMPK (A1/B1/G2), Active(P55-10H)

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Description :Recombinant full-length human AMPK (combination of A1/B1/G2 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tags.

Species :Human

Tag :HIS tags

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_006251

Genbank Number 2 :NM_006253

Genbank Number 3 :NM_001040633

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~68kDa (A1), ~38kDa (B1), ~65kDa (G2)

Gene Aliases :Subunit A1: PRKAA1, MGC33776, MGC57364 Subunit B1: PRKAB1, AMPK, HAMPKb, MGC17785 Subunit G2: PRKAG2, AAKG, CMH6, WPWS, AAKG2, H91620p

Scientific Background :AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) (2) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.

References :
1. Hardie, G.D. The AMP-activated protein kinase pathway – new players upstream and downstream. J. Cell Sci. 2004;117: 5479–5487.

2. Kahn, B.B. et al. AMP-activated protein kinase: Ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab; 2005: 1, 15–25.

F484-3.pdf

V2265-9.pdf

M2958-6.pdf

Research Areas :Cancer, Metabolic Disorder, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases, Cancer, Metabolic Disorder, Apoptosis/Autophagy, Cellular Stress, Ser/Thr Kinases