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Description :Recombinant human ALK4 (150-end) was expressed in Sf9 cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Genbank Number :NM_004302
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~64 kDa
Gene Aliases :ACVR1B, SKR2, ACTRIB, ACVRLK4
Scientific Background :ALK4 is a member of the subfamily of receptor ser/thr kinases that mediates signaling by the Activins. ALK4 is expressed in many human tissues, including kidney, pancreas, brain, lung, and liver. Truncated ALK4, predominantly expressed in human pituitary adenomas, function as dominant negative receptors to interfere with wild-type receptor function and blocks the antiproliferative effect of activin possibly contributing to development of human pituitary tumors (1). ALK4 is able to mediate Nodal signaling in the presence of Cripto during vertebrate development (2).
1. Zhou, Y. et al: Truncated activin type I receptor Alk4 isoforms are dominant negative receptors inhibiting activin signaling. Mol Endocrinol. 2000 Dec;14(12):2066-75.
2. Reissmann, E. et al: The orphan receptor ALK7 and the Activin receptor ALK4 mediate signaling by Nodal proteins during vertebrate development. Genes Dev. 2001 Aug 1;15(15):2010-22.
Formulation :50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Product Sheets (By Lot #) :
Research Areas :AKT/PKB Pathway, Angiogenesis, Cancer, Cardiovascular Disease, JAK/STAT Pathway, Neurobiology, Ser/Thr Kinases, Cancer, Neurobiology, Cardiovascular Disease, AKT/PKB Pathway, JAK/STAT Pathway, Angiogenesis, Ser/Thr Kinases