ALK1 siRNA Set I(A09-911)
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Description :ALK1 is a pool of three individual synthetic siRNA duplexes designed to knock-down human ALK1 mRNA expression. Each siRNA is 19-25 bases in length.
Specificity :ALK1 siRNAs are designed to specifically knock-down human ALK1 expression.
Formulation :The siRNAs are supplied as a lyophilized powder and shipped at room temperature.
Reconstitution Protocol :Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability :The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
Format :Lyophilized powder
Gene Aliases :ACVRL1, ACVRLK1, ALK1, HHT, HHT2, ORW2, SKR3, ALK1, TSR-I
Scientific Background :ALK1 is a serine/threonine-protein kinase receptor R3 precursor that mediates signal by TGF beta superfamily (1). ALK1 functions as a TGF beta type I receptor in endothelial cells and is responsible for human hereditary hemorrhagic telangiectasia (HHT) type II. Distinct Smad proteins (i.e., Smad2/Smad3 and Smad1/Smad5) show interaction with ALK1 and mediate TGF beta signaling. Northern blot and RT-PCR analysis show that ALK1 specifically induces expression of Smad6, Smad7, Id1, Id2, endoglin, STAT1, and interleukin 1 receptor in endothelial cells. ALK1 expression in inflammatory myofibroblastic tumor of the urinary bladder have also has been reported (2).
1. Ota, T. et al: Targets of transcriptional regulation by two distinct type I receptors for transforming growth factor-beta in human umbilical vein endothelial cells. J. Cell Physiol. 2002;193(3):299-318.
2. Tsuzuki, T. et al: ALK1 expression in inflammatory myofibroblastic tumor of the urinary bladder. Am. J. Surg. Pathol. 2004; 28(12):1609-14.
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Research Areas :AKT/PKB Pathway, Angiogenesis, Cancer, Cardiovascular Disease, JAK/STAT Pathway, Neurobiology, Ser/Thr Kinases, Cancer, Neurobiology, Cardiovascular Disease, AKT/PKB Pathway, JAK/STAT Pathway, Angiogenesis, Ser/Thr Kinases