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Description :Recombinant full-length human AKT3 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_005465
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~84 kDa
Gene Aliases :PKBG; PRKBG; STK-2; RAC-gamma; RAC-PK-gamma; DKFZP434N0250
Scientific Background :AKT 3 or Protein Kinase B γ (PKBγ) is a serine/threonine kinase that is a member of the AKT family. AKT 3 is activated in cells exposed to diverse stimuli such as hormones, growth factors, and extracellular matrix components (1). AKT 3 phosphorylates and regulates the function of many cellular proteins involved in processes that include cellular metabolism, survival/apoptosis, and proliferation. Recent evidence indicates that AKT 3 is frequently overexpressed in many types of human cancers including breast and prostate (2).
1. Coffer, PJ. et al: Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 (Pt 1): 1-13.
2. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.
Formulation :50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Product Sheets (By Lot #) :
Research Areas :Cancer, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, NfkB Pathway, WNT Signaling, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis, Ser/Thr Kinases, Cancer, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, NfkB Pathway, WNT Signaling, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis