AKT2/PKB beta siRNA Set I(A17-911)
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Description :AKT2/PKB beta is a pool of three individual synthetic siRNA duplexes designed to knock-down human AKT2/PKB beta mRNA expression. Each siRNA is 19-25 bases in length.
Specificity :AKT2/PKB beta siRNAs are designed to specifically knock-down human AKT2/PKB beta expression.
Reconstitution Protocol :Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability :The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
Format :Lyophilized powder
Gene Aliases :PRKBB; PKBBETA; RAC-BETA
Scientific Background :AKT2 or Protein Kinase B beta (PKB beta) is a serine/threonine kinase that is a member of the AKT family. AKT2 like the other AKT members is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility. The PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of AKT2 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1).
1. Coffer, PJ. et al: Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 335 (Pt 1): 1-13.
2. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.
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Research Areas :AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, Ser/Thr Kinases, WNT Signaling, Cancer, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, AKT/PKB Pathway, NfkB Pathway, WNT Signaling, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis