ATP-dependent DNA helicase RecQ, Active (AH01-E311H)

ATP-dependent DNA helicase RecQ, Active (AH01-E311H)

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Description : Recombinant full-length E. coli ATP-dependent DNA helicase RecQ was expressed in E. coli using C-terminal His-tag.

Species :Human

Tag :HIS tag

Expression System: E.Coli

Sequence : Full length

Genbank Number : WP_032255548.

ActivitySample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight :~70 kDa

Gene Aliases : RecQ

Scientific Background :  RecQ helicases catalyze double-stranded DNA unwinding in the 3’ to 5’ direction, which is driven by ATPase activity that is dependent on a bivalent cation (1).  RecQ helicases are important in coordinating genome maintenance and preserving genomic integrity in prokaryotes and eukaryotes (2). E. coli RecQ plays an important role in resuming DNA replication at stalled replication forks after repairing UV-induced DNA damage (3). They are also important in preventing future chromosomal abnormalities that result from DNA damage (3).

References :

  1. Sharma, S. et al: Mechanisms of RecQ helicases in pathways of DNA metabolism and maintenance of genomic stability. Biochem J, 2006; 398(3): 319-337.
  2. Bennet, R. J., Keck, J. L.: Structure and function of RecQ DNA helicases. Crit Rev Biochem Mol Biol., 2004; 39(2): 79-97.
  3. Hishida, T. et al: Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev., 2004; 18(15): 1886-1897.

Product Sheets (By Lot #) :

 T4216-5

 

Research Areas :Cancer, Neurobiology, Inflammation, Cell Cycle, Invasion/Metastasis, Cytoplasmic Tyrosine Kinases, Cancer, Neurobiology, Inflammation, Cell Cycle, Invasion/Metastasis, Cytoplasmic Tyrosine Kinases