AFX siRNA Set I(A42-911)
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Description :AFX is a pool of three individual synthetic siRNA duplexes designed to knock-down human AFX mRNA expression. Each siRNA is 19-25 bases in length.
Specificity :AFX siRNAs are designed to specifically knock-down human AFX expression.
Reconstitution Protocol :Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability :The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
Format :Lyophilized powder
Gene Aliases :FOXO4; AFX1; MLLT7
Scientific Background :AFX is a member of the O class of winged helix/forkhead transcription factor family. AFX proteins are involved in cell cycle regulation and inactivation of these proteins help in oncogenic transformation. AFX regulates apoptosis in part by suppressing the level of antiapoptotic BCLX through the transcriptional repressor BCL6 (1). AFX is down-regulated in patients with inflammatory bowel disease. AFX is expressed in human luteinized mural granulosa cells and may be involved in human folliculogenesis and luteinization. AFX acts as a novel anticancer agent for HER2-overexpressing cells.
1. Tang, T. et al: The forkhead transcription factor AFX activates apoptosis by induction of the BCL-6 transcriptional repressor. J. Biol. Chem. 27714255-14265, 2002
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Research Areas :Inflammation, Cellular Stress, Cell Cycle, Cancer, Apoptosis/Autophagy, AKT/PKB Pathway, Neurobiology, Cancer, Neurobiology, Cardiovascular Disease, Apoptosis/Autophagy