ADD1 siRNA Set I(A38-911)

ADD1 siRNA Set I(A38-911)

  • $595.00


Description :ADD1 is a pool of three individual synthetic siRNA duplexes designed to knock-down human ADD1 mRNA expression. Each siRNA is 19-25 bases in length.

Specificity :ADD1 siRNAs are designed to specifically knock-down human ADD1 expression.

Formulation :The siRNAs are supplied as a lyophilized powder and shipped at room temperature.

Reconstitution Protocol :Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.

Storage and Stability :The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.

Format :Lyophilized powder

Gene Aliases :alpha-adducin, ADDA, Erythrocyte adducin subunit alpha

Scientific Background :ADD1 or Adducin 1 is a heterodimeric protein associated with the erythrocyte membrane skeleton, which promotes binding of spectrin to actin, and is a substrate for PKC and PKA (1). Alpha- and gamma-adducins are ubiquitously expressed while beta-adducin is expressed at high levels in brain and hematopoietic tissues. ADD1 can completely block elongation and depolymerization at the fast-growing barbed ends of actin filaments in vitro and this suggests that adducin restrict actin filament length in erythrocytes (2).

References :
1. Gardner, K. A new erythrocyte membrane-associated protein with calmodulin binding activity: identification and purification. J. Biol. Chem. 261: 1339-1348, 1986.

2. Kuhlman. A new functions for adducin: calcium/calmodulin-regulated capping of the barbed ends of actin filaments. J. Biol. Chem. 271: 7986-7991, 1996.

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Research Areas :Neurobiology, Metabolic Disorder, Cardiovascular Disease, ERK/MAPK Pathway, NfkB Pathway, PKA/PKC Pathway, Apoptosis/Autophagy