ACTR2 Protein(A39-30G)

ACTR2 Protein(A39-30G)

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Description :Full-length recombinant human ACTR2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full length

Genbank Number :BC014546

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Western Blot

Molecular Weight :~70 kDa

Gene Aliases :ARP2

Formulation :50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Scientific Background :Belongs to the actin-related protein (Arp) superfamily whose primary sequences exhibited similarity, but not identity, to conventional actin. Along with Arp3 and 5 other proteins, ACTR2 makes up the evolutionary conserved Arp2/3 complex that was first identified in Acanthamoeba using profiling affinity chromatography and is an essential and ubiquitous component of the actin filament network. Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.

References :
1. Machesky LM, et al: 1994. Purification of a cortical actin complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose. J. Cell Biol. 127:107–15. 1994.

2. Schafer DA, et al: Actin-related proteins. Annu Rev Cell Dev Biol. 15:341-63. 1999

Product Sheets (By Lot #) :

U1763-7.pdf

Research Areas :Invasion/Metastasis, Cellular Stress, Cancer