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Description :Full-length recombinant human ACTR2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :BC014546
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~70 kDa
Gene Aliases :ARP2
Formulation :50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Scientific Background :Belongs to the actin-related protein (Arp) superfamily whose primary sequences exhibited similarity, but not identity, to conventional actin. Along with Arp3 and 5 other proteins, ACTR2 makes up the evolutionary conserved Arp2/3 complex that was ﬁrst identiﬁed in Acanthamoeba using proﬁling afﬁnity chromatography and is an essential and ubiquitous component of the actin filament network. Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
1. Machesky LM, et al: 1994. Puriﬁcation of a cortical actin complex containing two unconventional actins from Acanthamoeba by afﬁnity chromatography on proﬁlin-agarose. J. Cell Biol. 127:107–15. 1994.
2. Schafer DA, et al: Actin-related proteins. Annu Rev Cell Dev Biol. 15:341-63. 1999
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Research Areas :Invasion/Metastasis, Cellular Stress, Cancer