ACK siRNA Set I(A05-911)
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Description :ACK is a pool of three individual synthetic siRNA duplexes designed to knock-down human ACK mRNA expression. Each siRNA is 19-25 bases in length.
Specificity :ACK siRNAs are designed to specifically knock-down human ACK expression.
Formulation :The siRNAs are supplied as a lyophilized powder and shipped at room temperature.
Reconstitution Protocol :Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability :The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
Format :Lyophilized powder
Gene Aliases :TNK2, ACK1, FLJ44758, FLJ45547, p21cdc42Hs
Scientific Background :ACK is a tyrosine kinase that binds CDC42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of CDC42Hs (1). Overexpression of ACK in cancer cell lines of epithelial origin increases cellular motility and invasiveness. In a mouse model, ACK overexpression enhances the ability of a human breast cancer cell line to metastasize to the lung and increased mortality (2). Ligand stimulation of alpha-3 beta-1 integrin leads to activation of ACK which then enhances p130CAS phosphorylation and activation of RAC.
1. Manser, E. et al: A non-receptor tyrosine kinase that inhibits the GTPase activity of p21cdc42. Nature 363: 364-367, 1993.
2. Van der Horst, E. H.; Metastatic properties and genomic amplification of the tyrosine kinase gene ACK1. Proc. Nat. Acad. Sci. 102: 15901-15906, 2005.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cytoplasmic Tyrosine Kinases, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Neurobiology, Cancer, Neurobiology, Inflammation, ERK/MAPK Pathway, Invasion/Metastasis, Receptor Tyrosine Kinases