ACK, Active(A05-11G)

ACK, Active(A05-11G)

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Description :Recombinant human ACK (110-476) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :110-476

Genbank Number :NM_005781

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~66 kDa

Gene Aliases :TNK2, ACK1, FLJ44758, FLJ45547, p21cdc42Hs

Scientific Background :ACK is a tyrosine kinase that binds CDC42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of CDC42Hs (1). Overexpression of ACK in cancer cell lines of epithelial origin increases cellular motility and invasiveness. In a mouse model, ACK overexpression enhances the ability of a human breast cancer cell line to metastasize to the lung and increased mortality (2). Ligand stimulation of alpha-3 beta-1 integrin leads to activation of ACK which then enhances p130CAS phosphorylation and activation of RAC.

References :
1.Manser, E. et al: A non-receptor tyrosine kinase that inhibits the GTPase activity of p21cdc42. Nature 363: 364-367, 1993.

2.van der Horst, E. H.; Metastatic properties and genomic amplification of the tyrosine kinase gene ACK1. Proc. Nat. Acad. Sci. 102: 15901-15906, 2005.

Formulation :50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Product Sheets (By Lot #) :

H007-1.pdf

T990-2.pdf

Research Areas :Neurobiology, Invasion/Metastasis, Inflammation, ERK/MAPK Pathway, Cytoplasmic Tyrosine Kinases, Cancer, Cancer, Neurobiology, Inflammation, ERK/MAPK Pathway, Invasion/Metastasis, Receptor Tyrosine Kinases