ABL1 (F317I), Active(A03-12JG)

ABL1 (F317I), Active(A03-12JG)

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Description :Recombinant human ABL1 (F317I) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :27-end (F317I)

Genbank Number :NM_005157

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay

Molecular Weight :~160 kDa

Gene Aliases :ABL; JTK7; p150; c-ABL; v-abl; bcr/abl

Formulation :50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Scientific Background :ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of ABL protein is negatively regulated by its SH3 domain and deletion of the SH3 domain turns ABL1 into an oncogene (1). Translocation and head-to-tail fusion of the BCR and ABL1 genes is present in many cases of chronic myelogeneous leukemia (2). The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDK1-mediated phosphorylation, suggesting a cell cycle function for ABL1.

References :
1. Barila, D. et al: An intramolecular SH3-domain interaction regulates c-Abl activity. Nature Genet. 18: 280-282, 1998.

2. Goldman, J M. et al: Targeting the BCR-ABL tyrosine kinase in chronic myeloid leukemia. New Eng. J. Med. 344: 1084-1086, 2001.

Product Sheets (By Lot #) :

P1519-2.pdf

Q2510-11.pdf

Research Areas :Cancer, Cell Cycle, Cytoplasmic Tyrosine Kinases, Inflammation, Invasion/Metastasis, Neurobiology