Anti-Phospho-APC3 (Thr 244)(A89-365R)

Anti-Phospho-APC3 (Thr 244)(A89-365R)

  • $675.00


Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the Cdc27 protein phosphorylated at Threonine 244

Cited Applications :ELISA, IP, WB
Ideal working dilutions for each application should be empirically determined by the investigator.

Cross Reactivity :Human

Host Isotype / Clone# :Rabbit, IgG

Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 239-240 of Human Cdc27 protein.

Purification :Immunoaffinity chromatography

Stability :1yr at –20oC from date of shipment

Sample Data :Western blot using Affinity Purified anti-Phospho-APC3 (Thr244) (1:500) to detect phosphorylated human CDC27 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Each lane contains approximately 30µg of HeLa whole cell lysates.

Scientific Background :Cdc27 (also known as cell division cycle protein 27 homologue, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2.  Cdc27 is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.  Cdc27 has a nuclear localization.

References :
1. Gabellini,D., et al.: Early mitotic degradation of the homeoprotein HOXC10 is potentially linked to cell cycle progression. EMBO J. 2003: 22 (14); 3715-3724.

2. Topper,L.M., et al. The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis. Cell Cycle. 2002:1 (4); 282-292.

3. Wassmann,K., et al.: Mad2 transiently associates with an APC/p55Cdc complex during mitosis. Proc. Natl. Acad. Sci. U.S.A. 1988: 95 (19); 11193-11198.

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Research Areas :Cancer, Cell Cycle, Neurobiology, p38 Pathway