Anti-Phospho-APC3 (Ser427)(A89-365BR)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the human Cdc27 protein phosphorylated at Serine 427.
Cited Applications :ELISA, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human, Rat, Dog, Chicken and Chimpanzee
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 422-430 of human Cdc27 protein.
Purification :Immunoaffinity chromatography
Stability :1yr at –20oC from date of shipment
Scientific Background :Cdc27 (also known as cell division cycle protein 27 homologue, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. Cdc27 is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Cdc27 has a nuclear localization.
References :
1. Gabellini,D., et al.: Early mitotic degradation of the homeoprotein HOXC10 is potentially linked to cell cycle progression. EMBO J. 2003: 22 (14); 3715-3724.
2. Topper,L.M., et al. The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis. Cell Cycle. 2002:1 (4); 282-292.
3. Wassmann,K., et al.: Mad2 transiently associates with an APC/p55Cdc complex during mitosis. Proc. Natl. Acad. Sci. U.S.A. 1988: 95 (19); 11193-11198.
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Research Areas :Cancer, Cell Cycle, Neurobiology, p38 Pathway