Anti-Phospho-APC1 (Ser377)(A87-365R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the APC1 protein phosphorylated at Serine 377
Cited Applications :ELISA, IHC, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 373-382 of human APC1 protein.
Purification :Immunoaffinity chromatography followed by solid phase adsorption.
Stability :1yr at –20oC from date of shipment
Sample Data :Western blot using Anti-Phospho-APC1 (Ser377) (1:1,000) shows detection of a band ~215 kDa corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Each lane contains approximately 30 µg of HeLa whole cell lysates, separated by 4-8% SDS-PAGE followed by transfer to nitrocellulose.
Sample Data :Anti-Phospho-APC1 (Ser377) antibody was used at 5.0 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides at 60X. Tissue was formalin-fixed and paraffin embedded.
Scientific Background :APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.
References :
1. Jorgensen,P.M., et al.: Characterization of the human APC1, the largest subunit of the anaphase-promoting complex. Gene. 2001;262 (1-2): 51-59.
2. Kraft C, et al.: Mitotic regulation of the human anaphase-promoting complex by phosphorylation. Embo J. 2003;22(24):6598-609
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Research Areas :Cancer, Cell Cycle, Neurobiology, p38 Pathway