Anti-Phospho-AURORA B (Thr 232)(A31-65R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the human Aurora Kinas B protein phosphorylated at Threonine 232.
Cited Applications :ELISA, IF, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human and Mouse
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 223-234 of Human Aurora Kinase B protein.
Purification :Affinity chromatography
Stability :1yr at –20oC from date of shipment
Sample Data :Western Blot detection of Aurora B protein at 39 using Anti-Phospho-AURORA B (Thr2320 antibody (1:500). Lane 1 : Extract from COS7 cells treated with Nocodazole (1µg/ml, 16 hrs). Lane 2: Extract from COS7 cells treated with Nocodazole (1µg/ml, 16 hrs) and with the phosphopeptide immunogen.
Sample Data :Immunohistochemical analysis of formalin fixed, paraffin embedded human intestine (pH9) tissue (A) at 20x using 10 µg/mL Anti-phospho-AURORA B (Thr232). Analysis of formalin fixed, paraffin embedded human placenta pH9 tissue (A) at 20x using 10 µg/mL Anti-Phospho-AURORA B (Thr232)
Scientific Background :AURORA B is a member of the Aurora kinase family that associates with microtubules during chromosome movement and segregation. AURORA B localizes to the microtubules near kinetochores, specifically to the specialized microtubules called K-fibers (1). AURORA B inhibits the microtubule depolymerizing activity of mitotic centromere-associated kinesin (MCAK) by phosphorylating MCAK on Ser92 (2). This phosphorylation also regulates MCAK translocalization from kinetochores to the centromere. AURORA B has been identified as a target for the development of new anticancer agents since inhibition of AURORA B gives rise to the more pronounced antiproliferative phenotype.
References :
1. Shannon, K B. et al: Chromosome dynamics: new light on Aurora B kinase function. Curr Biol. 2002 Jul 9;12(13):R458-60.
2. Andrews, P. D. et al: Aurora B regulates MCAK at the mitotic centromere. Dev. Cell 6: 253-268, 2004.
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Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases