Aurora B, Active (A31-10H)
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Description : Recombinant full-length human Aurora B was expressed by baculovirus in Sf9 insect cells using an N-terminal His(8) tag.
Expression System: Sf9 insect cells
Gene Aliases: AURKB, AIK2; AIM1; ARK2; AurB; IPL1; AIM-1; STK12
Formulation: Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
Sequence : Full length
Storage and Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background : AURORA B is a member of the Aurora kinase family that associates with microtubules during chromosome movement and segregation. AURORA B localizes to the microtubules near kinetochores, specifically to the specialized microtubules called K-fibers (1). AURORA B inhibits the microtubule depolymerizing activity of mitotic centromere-associated kinesin (MCAK) by phosphorylating MCAK on Ser92 (2). This phosphorylation also regulates MCAK translocalization from kinetochores to the centromere. AURORA B has been identified as a target for the development of new anticancer agents since inhibition of AURORA B gives rise to the more pronounced antiproliferative phenotype.
References :
1. Shannon, K B. et al: Chromosome dynamics: new light on Aurora B kinase function. Curr Biol. 2002 Jul 9;12(13):R458-60.
2. Andrews, P. D. et al: Aurora B regulates MCAK at the mitotic centromere. Cell 6: 253-268, 2004.
Product Sheets (By Lot #) : Y4435-2
Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases