Anti-Phospho-ATM (Ser1981)(A26-65S)
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Description :Sheep Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the ATM protein phosphorylated at Serine 1981
Cited Applications :ELISA, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human
Host Isotype / Clone# :Sheep, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to a region near serine 1981 of human ATM conjugated to KLH.
Purification :Affinity chromatography
Stability :1yr at –20oC from date of shipment
Sample Data :Western Blot of Anti-Phospho-ATM (Ser1981) antibody (1:1,000). Lane 1: untreated MCF-7 cell lysate. Lane 2: Hydrogen Peroxide stimulated MCF-7 Whole Cell Lysate. Load: 35 µg per lane.
Scientific Background :ATM is a master regulator for dsDNA break repair, which belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily. Unlike other lipid kinases, ATM phosphorylates proteins. ATM is present in undamaged cells as an inactive dimer, which is activated likely in response to changes in chromatin structure. Exposure of cells to ionizing radiation triggers ATM kinase activity, leading to cell cycle arrest in the G1, S or G2 phase. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, MDM2 and CHK2 in the G1 checkpoint; NBS1, BRCA1, FancD2 and SMC1 in the transient IR-induced S-phase arrest; and BRCA1 and hRAD17 during the G2/M checkpoint. ATM is mutated in the hereditary disease ataxia-telangiectasia.
References :
1. Bakkenist, C. J., et al.: DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. Nature. 2003: 421; 499-506.
2. Kitagawa R, et al.: Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway. Genes Dev. 2004: 18(12);1423-38.
3. Falck, J. et al.: Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nature. 2005: 434; 605-611.
Product Sheets (By Lot #) :
Research Areas :Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Inflammation, Lipid Kinases, Metabolic Disorder, Ser/Thr Kinases