ALK (I1171N D1203N), Active(A19-12PG)
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Description :Recombinant human ALK (I1171N D1203N) (1060-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST
Expression System:Sf9 insect cells using baculovirus
Sequence :1060-end
Genbank Number :NM_004304
Alternative Name(s) :ALK (Ki-1), CD246, NBLST3, TFG/ALK
Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 300mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :ALK or anaplastic lymphoma kinase is a receptor tyrosine kinase that was originally identified as a member of the insulin receptor subfamily that acquires transforming capability when truncated and fused to NPM (nucleophosmin) in the t (2; 5) chromosomal rearrangement associated with ALCL Many chromosomal rearrangements leading to enhanced ALK activity have been described and are implicated in a number of cancer types (1). In the nervous system, ALK in the presence of ligand appears essential for axonal guidance, whereas in the absence of ligand, ALK expression can lead to developmental neuronal apoptosis (2).
References :1. Palmer R H, et al: Anaplastic lymphoma kinase: signalling in development and disease. Biochem J. 2009 May 27; 420(3): 345-61.
2. Allouche M: ALK is a novel dependence receptor: potential implications in development and cancer. Cell Cycle. 2007 Jul 1;6(13):1533-8
Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Approx. MW 90kDa.
Product Sheets (By Lot #) :
X3684-5.pdf K4502-1.pdf Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle