Anti-phospho-AKT1/2/3 (Ser473)(A16-65BM)
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Description :Mouse Monoclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the AKT protein phosphorylated at Serine 473
Cited Applications :ELISA, IHC, IF, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human, Mouse, Rat and Monkey
Host Isotype / Clone# :Mouse, IgG1
Immunogen :The antibody was produced against synthesized peptide corresponding to residues surrounding Ser 473 of human AKT1 protein.
Purification :Protein A Chromatography
Stability :1yr at –20oC from date of shipment
Sample Data :Immunohistochemical analysis of formalin fixed, paraffin embedded human prostate tissue using Anti-Phospho-AKT1/2/3 (Ser473)
Sample Data :Western Blot of Mouse Anti-AKT pS473 antibody (1:10,000 in TBS with 0.05% Tween-20 with 1% BSA). Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane.
Scientific Background :AKT1/PKBα is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (2).
References :
1. Coffer, PJ. et al: Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 1998 Oct 1; 369 ( Pt 1):1-13.
2. Anderson, KE. et al: Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol. 1998 Jun 4;8(12): 684-91.
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Research Areas :AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling