ATG4B Protein (A104-34G)
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Description :Recombinant full-length human ATG4B was expressed in E. coli cells using an N-terminal His tag.
Species :Human
Tag :HIS
Expression System:E.coli
Sequence :Full length
Genbank Number :Q9Y4P1
Alternative Name(s) :Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; AutoAPG4B; AUTL1; KIAA0943
Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :ATG4B is a cysteine protease that plays a crucial role in the process of autophagy, which is a cellular mechanism for degradation and recycling of intracellular components. ATG4B is one of four mammalian orthologs of the yeast protein Atg4, which cleaves the C-terminal region of the Atg8 protein to expose a glycine residue that is required for its conjugation to phosphatidylethanolamine during autophagosome formation. In mammals, ATG4B has been shown to be involved in the processing of several Atg8 homologs, including LC3B and GABARAPL1, which are essential for the formation and maturation of the autophagosome.
References :1. Marino, G. et al: Self-consumption: the interplay of autophagy and apoptosis. Nat Rev Mol Cell Biol. 2014;15(2):81-94. doi:10.1038/nrm3735.
2. Mizushima, N. et al: Methods in mammalian autophagy research. Cell. 2010;140(3):313-326. doi:10.1016/j.cell.2010.01.028.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :Observed MW ~52 kDa. Calculated MW ~46 kDa.
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