Anti-phospho-ATF2 (Ser490 Ser498)(A10-565BR)

Anti-phospho-ATF2 (Ser490 Ser498)(A10-565BR)

  • $715.00


Description :Rabbit Polyclonal Antibody

Species :Rabbit

Tag :

Expression System:

Sequence :

Specificity :Recognizes the ATF2 protein phosphorylated at serine 490/498

Cited Applications :WB, IHC

Cross Reactivity :Human and Rat

Host :Rabbit, IgG

Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser490/498 conjugated to KLH

Purification :Affinity Chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immunolabeling of the ~74kDa ATF2 protein phosphorylated at Ser490 and Ser498.

Scientific Background :The activating transcription factor ATF2 (also called CRE-BP1) is a member of the ATF/CREB family of leucine zipper transcription factors, which binds both AP-1 and Cre DNA response element (1). ATF2 has two unique characteristics: it mediates adenovirus E1A-induced trans-activation and forms a heterodimer with c-Jun (2). ATF2 is a target of the JNK signal transduction pathway (3). ATF2 is phosphorylated by JNK on two closely spaced threonine residues located in the NH2-terminal activation domain. The replacement of these phosphorylation sites with alanine inhibited the transcriptional activity of ATF2. Furthermore, p38 MAPK can also phosphorylate ATF2.

References :
1. Maekawa, T. et al: Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain. EMBO J 1989 8:2023-2028.

2. Nomura, N. et al: Isolation and characterization of a novel member of the gene family encoding the cAMP response element binding protein CRE-BP1. J Biol Chem. 1993 Feb 25;268(6):4259-66.

3. Gupta, S. et al: Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science, 1995 Jan 20;267(5196):389-93.

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Research Areas :Neurobiology, Metabolic Disorder, Cardiovascular Disease, p38 Pathway, NfkB Pathway, Apoptosis/Autophagy, Cellular Stress, Angiogenesis