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Description : Epac pathway, RAP1 pathway activator
CAS Registry Number: 1152197-23-3
Molecular Formula : C20H21CIN5O8PS
Appearance : White lyophilized solid
Melting Point : N/A
Molecular Weight : 557.9
Purity : >97% (HPLC)
Solubilization : May be dissolved in DMSO (50 mg/ml). Poorly soluble in aqueous solution.
Aliases : 8-(4-Chlorophenylthio)-2'-O-methyladenosine-3', 5'-cyclic monophosphate acetoxymethyl ester
Storage and Stability : Store desiccated as supplied at -20oC for up to 2 years. Store solutions at -20oC for up to 1 month.
Scientific Background : A potent, cell permeable Epac (exchange protein directly activated by cAMP) activator (1). Induces RAP1 activation and insulin secretion in pancreatic beta cell lines (2-5). Induces vascular relaxation in rate mesenteric artery (6). The acetoxymethyl ester confers increased cell-permeability and is cleaved by endogenous esterases to yield the active compound, 8-pCPT-2’-O-Me-cAMP. Addition to cell cultures should be done in serum-free media as esterases in the serum will cleave the acetoxymethyl ester and reduce cell permeability.
1. MJ Vliem et al. Chembiochem. Sci.2008 9:2052.
2. OG Chepurny et al. J. Biol. Chem. 2009 284:10726.
3. GG Kelley et al. Islets 2009 1:260.
4. OG Chepurny et al. Am. J. Physiol. Edocrinol. Metab. 2010 298:E622.
5. I Dzhura et al Islets 2011 3:121.
6. OL Roberts et al. J. Physiol. 2013 591:5107.
Molecular Structure : Sample Purity Data. For specific information on a given lot, see related technical data sheet.
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Research Areas: AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Cytoplasmic Tyrosine Kinases, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, JAK/STAT Pathway, JNK/SAPK Pathway, Metabolic Disorder, Neurobiology, NfkB Pathway, p38 Pathway, PKA/PKC Pathway, Receptor Tyrosine Kinases, Ser/Thr Kinases, WNT Signaling,