4EBP1 siRNA Set I(E35-911)
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Description :4EBP1 is a pool of three individual synthetic siRNA duplexes designed to knock-down human 4EBP1 mRNA expression. Each siRNA is 19-25 bases in length.
Specificity :4EBP1 siRNAs are designed to specifically knock-down human 4EBP1 expression.
Reconstitution Protocol :Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability :The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
Format :Lyophilized powder
Gene Aliases :BP-1, EIF4EBP1, PHAS-I, MGC4316
Scientific Background :4EBP1 is a member of a family of translation repressor proteins that directly interact with eukaryotic translation initiation factor 4E (EIF4E). Interaction of 4EBP1 with EIF4E inhibits the multi-subunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs thereby leading to repression of translation. Insulin treatment of adipose cells increases the phosphorylation of 4EBP1 and leads to reduced interaction of 4EBP1 with EIF4E (1). 4EBP1 is expressed in most tissues, with highest levels seen in adipose tissue, pancreas, and skeletal muscle (2).
1. Pause, A. et al: Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5-prime-cap function. Nature371: 762-767, 1994.
2. Tsukiyama-Kohara, K. et al: Tissue distribution, genomic structure, and chromosome mapping of mouse and human eukaryotic initiation factor 4E-binding proteins 1 and 2. Genomics38: 353-363, 1996.
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Research Areas :AKT/PKB Pathway, Angiogenesis, Cancer, Metabolic Disorder, Cancer, Metabolic Disorder, AKT/PKB Pathway, Angiogenesis