2019-nCoV 3CLpro, Active (C19CL-G241H)

2019-nCoV 3CLpro, Active (C19CL-G241H)

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Description :Recombinant full-length 2019-nCoV 3CLpro, part of a large replicase polyprotein 1ab (S3264-Q3569), was expressed in E. coli cells with an N-terminal His tag.

Species :Virus

Tag :HIS tag

Expression System:CHO cells

Sequence :Full length

Genbank Number :QHD43415

Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight :35 kDa

Gene Aliases :SARS-CoV-2 3CLpro; NSP5; Mpro

Scientific Background :SARS-CoV-2 3C-like proteinase (3CLpro) is the nonstructural protein number 5, also a cysteine protease, that is released from the virally encoded polyprotein by extensive proteolytic processing. The 3CLpro cleaves the C-terminus of the large replicase polyprotein 1ab (~790 kDa) at no fewer than 11 sites (1). Inhibiting the activity of this enzyme would block viral replication (2). The functional importance of 3CLpro in the viral life cycle makes this protease an attractive target for the development of drugs directed against SARS and other coronavirus infections (3).

References :
1. Hegyi, A. et al: Conservation of substrate specificities among coronavirus main proteases. J Gen Virol. 2002,83:595-599.

2. Zhang L. et al: Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved α-ketoamide inhibitors. Science. 2020, 368:409-412.

3. Anand K. et al: Coronavirus main proteinase (3CLpro) structure: Basis for design of anti-SARS drugs. Science. 2003, 300:1763-1767.

Product Sheets (By Lot #) :

S3702-12b.pdf