14-3-3 theta siRNA Set I(Y84-911)
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Description :14-3-3 theta is a pool of three individual synthetic siRNA duplexes designed to knock-down human 14-3-3 theta mRNA expression. Each siRNA is 19-25 bases in length.
Specificity :14-3-3 theta siRNAs are designed to specifically knock-down human 14-3-3 theta expression.
Reconstitution Protocol :Each siRNA duplex is supplied as 2.5nmol per tube (for example 6 x 2.5nmol = 3 x 5nmol per duplex). Briefly centrifuge one tube per duplex (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend each 2.5nmol tube in 50 ul of DEPC-treated water (included in kit), which results in a 1x stock solution (50 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability :The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
Format :Lyophilized powder
Gene Aliases :14-3-3 theta, YWHAQ, 1C5, HS1, 14-3-3
Scientific Background :14-3-3 theta (also known as tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activation protein, theta polypeptide) is a member of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. Through interaction with ASK1, c-jun NH-terminal kinase, and p38 mitogen-activated protein kinase (MAPK), 14-3-3 theta plays an important role in controlling apotopsis (1). Induced expression of 14-3-3 theta protein has been reported in patients with amyotrophic lateral sclerosis. Additionally, 14-3-3 theta has been observed to mediate nucleo-cytoplasmic shuttling of the N protein (coronavirus nucleocapsid protein) which causes severe acute respiratory syndrome (2).
1. Lau, J.M. et al: The 14-3-3tau phosphoserine-binding protein is required for cardiomyocyte survival. Mol Cell Biol. 2007, 27(4):1455-66.
2. The severe acute respiratory syndrome coronavirus nucleocapsid protein is phosphorylated and localizes in the cytoplasm by 14-3-3-mediated translocation.J Virol. 2005 Sep;79(17):11476-86.
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Research Areas :AKT/PKB Pathway, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, WNT Signaling, Cancer, Neurobiology, AKT/PKB Pathway, ERK/MAPK Pathway, PKA/PKC Pathway, WNT Signaling, Cell Cycle, Cellular Stress