14-3-3 theta Protein(Y84-30H)
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Description :Recombinant full-length human 14-3-3 theta was ex-pressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Tag :His tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_006826
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :32 kDa
Gene Aliases :YWHAQ, 1C5, HS1, 14-3-3
Formulation :50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Scientific Background :14-3-3 theta (also known as tyrosine 3-monooxygenase /tryptophan 5-monooxygenase activation protein, theta polypeptide) is a member of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. Through interaction with ASK1, c-jun NH-terminal kinase, and p38 mitogen-activated protein kinase (MAPK), 14-3-3 theta plays an important role in controlling apotopsis (1). Induced expression of 14-3-3 theta protein has been reported in patients with amyotrophic lateral sclerosis. Additionally, 14-3-3 theta has been observed to mediate nucleo-cytoplasmic shuttling of the N protein (coronavirus nucleocapsid protein) which causes severe acute respiratory syndrome (2).
1. Lau, J.M. et al: The 14-3-3tau phosphoserine-binding protein is required for cardiomyocyte survival. Mol Cell Biol. 2007, 27(4):1455-66.
2. The severe acute respiratory syndrome coronavirus nucleocapsid protein is phosphorylated and localizes in the cytoplasm by 14-3-3-mediated translocation.J Virol. 2005 Sep;79(17):11476-86.
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Research Areas :AKT/PKB Pathway, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, WNT Signaling