14-3-3 sigma siRNA Set I(Y86-911)
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Description :14-3-3 sigma is a pool of three individual synthetic siRNA duplexes designed to knock-down human 14-3-3 sigma mRNA expression. Each siRNA is 19-25 bases in length.
Specificity :14-3-3 sigma siRNAs are designed to specifically knock-down human 14-3-3 sigma expression.
Formulation :The siRNAs are supplied as a lyophilized powder and shipped at room temperature.
Reconstitution Protocol :Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.
Storage and Stability :The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated Handling and multiple freeze/thaw cycles.
Format :Lyophilized powder
Gene Aliases :14-3-3 sigma, SFN, stratifin, YWHAS
Scientific Background :14-3-3 sigma or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents (1). The induction of 14-3-3 sigma is mediated by a p53 responsive element. Exogenous introduction of 14-3-3 sigma into cycling cells results in a G2 cell cycle arrest (2). Knockout of 14-3-3 sigma in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3 sigma -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3 sigma -deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3 sigma plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death.
1. Chan, T A. et al : 14-3-3-sigma is required to prevent mitotic catastrophe after DNA damage. Nature 401:616-620, 1999.
2. Hermeking, H. et al: 14-3-3-sigma is a p53-regulated inhibitor of G2/M progression. Molec. Cell 1:3-11, 1997.
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Research Areas :AKT/PKB Pathway, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, WNT Signaling, Cancer, Neurobiology, AKT/PKB Pathway, ERK/MAPK Pathway, PKA/PKC Pathway, WNT Signaling, Cell Cycle, Cellular Stress