14-3-3 sigma Protein(Y86-30N)
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Description :Recombinant full-length human tag-free 14-3-3sigma was expressed in E. coli.
Sequence :Full Length
Genbank Number :NM_006142
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~29 kDa
Gene Aliases :14-3-3 sigma, SFN, stratifin, YWHAS
Scientific Background :14-3-3σ or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents (1). The induction of 14-3-3σ is mediated by a p53 responsive element. Exogenous introduction of 14-3-3σ into cycling cells results in a G2 cell cycle arrest (2). Knockout of 14-3-3σ in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3σ -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3σ-deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3σ plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death.
1. Chan, T A. et al : 14-3-3-sigma is required to prevent mitotic catastrophe after DNA damage. Nature 401: 616-620, 1999.
2. Hermeking, H. et al: 14-3-3-sigma is a p53-regulated inhibitor of G2/M progression. Molec. Cell1: 3-11, 1997.
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Research Areas :Cancer, Neurobiology, AKT/PKB Pathway, ERK/MAPK Pathway, PKA/PKC Pathway, WNT Signaling, Cell Cycle, Cellular Stress, Cancer, Neurobiology, AKT/PKB Pathway, ERK/MAPK Pathway, PKA/PKC Pathway, WNT Signaling, Cell Cycle, Cellular Stress