14-3-3 sigma Protein(Y86-30H)
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Description :Recombinant full-length human 14-3-3 sigma was ex-pressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Tag :His tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_006142
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :30 kDa
Gene Aliases :14-3-3 sigma, SFN, stratifin, YWHAS
Formulation :50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol
Scientific Background :14-3-3 sigma or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents (1). The induction of 14-3-3 sigma is mediated by a p53 responsive element. Exogenous introduction of 14-3-3 sigma into cycling cells results in a G2 cell cycle arrest (2). Knockout of 14-3-3 sigma in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3sigma -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3sigma-deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3 sigma plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death.
1. Chan, T A. et al: 14-3-3-sigma is required to prevent mitotic catastrophe after DNA damage. Nature 401: 616-620, 1999.
2. Hermeking, H. et al: 14-3-3-sigma is a p53-regulated inhibitor of G2/M progression. Molec. Cell 1: 3-11, 1997.
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Research Areas :AKT/PKB Pathway, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, WNT Signaling