UBA1 (UBE1), Active(U201-380G)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Full-length recombinant human UBA1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_003334
Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Ubiquitin Enzyme Assay, Western Blot
Molecular Weight :145 kDa
Gene Aliases :UBE1, CTD-2522E6.1, A1S9, A1S9T, A1ST, AMCX1, GXP1, POC20, SMAX2, UBA1A, UBE1X
Scientific Background :Ubiquitin-activating enzyme 1 (UBA1) catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. UBA1 activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Posttranslational modification by ubiquitin or ubiquitin-like proteins regulates numerous processes, including cell division, immune responses and embryonic development.
References :
1. Mondal, S. et al: A bioluminescent assay for monitoring conjugation of ubiquitin and ubiquitin-like proteins. Anal. Biochem. 510: 41-51, 2016
2. Haas, A.L. et al: Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation. J. Biol. Chem. 257(5):2543-2548, 1982
3. McGrath, J.P. et al: UBA 1: an essential yeast gene encoding ubiquitin-activating enzyme. EMBO J. 10(1):227-236, 1991
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle, Neurobiology