KRAS (G12C) Protein (R06-32DH)
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Description :Recombinant human KRAS (G12C) (2-186) was expressed in E.coli cells using an N-terminal His tag. The KRAS (G12C) mutant is constitutively active.
Species :Human
Tag :His tag
Expression System:E.coli
Sequence :2-186
Genbank Number :NM_033360
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Enzyme Assay, Western Blot
Molecular Weight :23 kDa
Gene Aliases :C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2
Scientific Background :The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking (1). There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and K-RAS occur in varying frequencies in different tumor types (2). Other members of the RAS superfamily may also contribute to cancer.
References :
1.Shih, T. Y., et al: Structure and function of p21 ras proteins. Gene Amplif Anal. 1986;4:53-72.
2.Rodriguez-Viciana, P.: Cancer targets in the Ras pathway. Cold Spring Harb Symp Quant Biol. 2005;70:461-7.
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Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology