KEX2 Protease, Active(K525-31N)

KEX2 Protease, Active(K525-31N)

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Description :Recombinant saccharomyces cerevisiae Kex-2 (110-660aa) protease was expressed in yeast cells.

Species :Yeast

Tag :tag-free

Expression System:Yeast cells

Sequence :110-660 aa

Sample Data :

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Enzyme Commission Number :EC 3.4.21.61

Gene Aliases :Kexin; YSCF; Kex-2

Storage, Stability, and Shipping :Store product at –70oC for up to 1 year. Aliquot enzymes to avoid freeze / thaw cycles.

Applications :In vitro Proteolysis

Digestion Conditions :Stable pH range: 5.0~6.0
Optimum catalytic pH: 9.0
Optimum catalytic temperature: 37oC

Scientific Background :Yeast KEX2 is a calcium-dependent serine protease that shares structural similarities with the bacterial protease subtilisin. KEX2 hydrolyzes the bond between Lys-Arg, Arg-Arg, Pro-Arg amino acid pairs. Recombinant KEX2 is useful for processing fusion proteins that contain the KEX2 recognition site. The enzyme stabilizes in a pH range of 5.0-6.0. The optimum catalytic pH is 9.0. The enzyme is not inhibited by conventional serine protease inhibitors such as PMSF, TPCK and TLCK.

Product Sheets (By Lot #) :

L2146-4.pdf