PDE8A, Active(P96-30G)
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Description :Full-length recombinant human PDE8A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_002605
Alternative Name(s)
FLJ16150; HsT19550
Storage and Stability
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background
PDE8A is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP and cGMP. PDE8A is a high-affinity cAMP-specific PDE expressed in a variety of tissues such as ovary and testis. PDE8A plays an important role in many biological processes, including T-cell activation, testosterone production, adrenocortical hyperplasia, and thyroid function. Targeted mutation in the PDE8A gene in Leydig cells show sensitivity in the action of LH in terms of testosterone production (1). PDE8A, through its PAS domain, can bind with IkappaB proteins in a region containing their ankyrin repeats. The association of PDE8A with IkappaB greatly enhanced the enzyme activity this enzyme (2).
References
- Chen C, et al: PDE8A genetic variation, polycystic ovary syndrome and androgen levels in women. Mol Hum Reprod. 2009 Aug;15(8):459-69.
- Wu P, et al: Per-Arnt-Sim domain-dependent association of cAMP-phosphodiesterase 8A1 with IkappaB proteins. Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17634-9.
Product Sheets (By Lot #) :
Research Areas :Inflammation, Cardiovascular Disease, Phosphodiesterases