PP2A alpha/PPP2R1A Complex, Active(P16-20BH)
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Description :Recombinant full length human PP2Aalpha and human PPP2R1A were expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Species :Human
Tag :His tags
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_002715
Genbank Number 2 :NM_014225
Specific Activity :Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Phosphatase Assay, Western Blot
Molecular Weight :PP2Aalpha ~38 kDa and PPP2R1A ~65 kDa
Gene Aliases :PP2Aalpha: RP-C; PP2Ac; PP2CA; PPP2CA
PPP2R1A: MRD36; PP2A-Aalpha; PP2AAALPHA; PR65A
Scientific Background :PP2Aalpha is also known as Protein phosphatase 2A active subunit. PP2A is one of the four major Ser/Thr phosphatases and is a human tumor suppressor that inhibits cellular transformation. PP2A alpha gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase-2A (1). Protein phosphatase 2A holoenzyme is a heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. PTP2A alpha can alter the substrate specificity of PP2A leading to enhanced phosphotyrosine phosphatase activity and decreased phosphoserine phosphatase activity (2).
References :
1. Van Hoof, C. et.al: Structure and chromosomal localization of the human gene of the phosphotyrosyl phosphatase activator (PTPA) of protein phosphatase 2A. Genomics 28: 261-272, 1995.
2. Chao, Y. et.al: Structure and mechanism of the phosphotyrosyl phosphatase activator. Molec. Cell 23: 535-546, 2006.
Product Sheets (By Lot #) :
Research Areas :AKT/PKB Pathway, Cancer, Cell Cycle, Inflammation, Metabolic Disorder, Neurobiology, Phosphatases