Vimentin Protein(V49-30H)
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Description :Recombinant full-length human Vimentin was expressed in E. coli cells using an N-terminal His tag.
Species :Human
Tag :HIS tag
Expression System:E.coli
Sequence :Full Length
Genbank Number :NM_003380
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.gel.gif)
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~60 kDa
Gene Aliases :VIM, FLJ36605
Scientific Background :Vimentin is a member of intermediate filament family of proteins and is an important structural feature of eukaryotic cells (1). Along with microtubules and actin microfilaments, Vimentin make up the cytoskeleton component of cells. Studies have shown that Vimentin is attached to the nucleus, endoplasmic reticulum and mitochondria, either laterally or terminally. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol (2). Thus, Vimentin plays a key role in maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions
References :
1. Fuchs, E., et al: Intermediate filaments: structure, dynamics, function, and disease". Annu Rev Biochem,1994; 63: pp. 345–82.
2. Katsumoto, T. et al: The role of the vimentin intermediate filaments in rat 3Y1 cells elucidated by immunoelectron microscopy and computer-graphic reconstruction. Biol Cell,1990; 68 (2): pp. 139–46.
Product Sheets (By Lot #) :
Research Areas :Cancer, Neurobiology, Inflammation, Cardiovascular Disease, Apoptosis/Autophagy, Cell Cycle, Cellular Stress, Invasion/Metastasis, Cancer, Neurobiology, Inflammation, Cardiovascular Disease, Apoptosis/Autophagy, Cell Cycle, Cellular Stress, Invasion/Metastasis