PNMT, Active(P331-380G)
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Description :Full-length recombinant human PNMT was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_002686
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Methyltransferase Assay
Molecular Weight :57 kDa
Gene Aliases :PENT; PNMTase
Scientific Background :Phenylethanolamine N-methyltransferase (PNMT) catalyzes the biosynthesis of adrenaline though methylation of phenylethanolamine (1). PNMT gene is reported to be involved in various human diseases including early-onset Alzheimer disease (2), Parkinson's disease (3), and multiple sclerosis (4). Expression of PNMT can be stimulated by nicotine (5).
References :
1. Kaneda, N., et al. Molecular cloning of cDNA and chromosomal assignment of the gene for human phenylethanolamine N-methyltransferase, the enzyme for epinephrine biosynthesis. J Biol Chem. 263(16):7672-7, 1988.
2. Mann, M.B., et al. Phenylethanolamine N-methyltransferase (PNMT) gene and early-onset Alzheimer disease. Am J Med Genet. 105(4):312-6, 2001.
3. Gearhart, D.A., et al. Phenylethanolamine N-methyltransferase has beta-carboline 2N-methyltransferase activity: hypothetical relevance to Parkinson's disease. Neurochem Int. 40(7):611-20, 2002.
4. Mann, M.B., et al. Association between the phenylethanolamine N-methyltransferase gene and multiple sclerosis. J Neuroimmunol. 124(1-2):101-5, 2002.
5. Evinger, M.J., et al. Nicotine stimulates expression of the PNMT gene through a novel promoter sequence. J Mol Neurosci. 26(1):39-55, 2005.
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Research Areas :Cardiovascular Disease, Metabolic Disorder, Neurobiology