DGKH, Active (D26-10G)
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Description :Recombinant full-length human DGKH was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species : Human
Tag : N Terminal GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_178009.5
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications: Kinase Assay
Molecular Weight: 165 kDa
Gene Aliases: Diacylglycerol kinase eta; Diglyceride kinase eta
Scientific Background: DGKH is a type II DGK encoded by gene DGKH (1). Diacylglycerol kinases modulate the concentration of intracellular diacylglycerol by phosphorylating, producing phosphatidic acid (PA) (2). PA regulates signaling proteins, such as protein kinases and phosphatases, and can induce cell mitosis through regulation of growth factors (3). DGKH is a positive regulator of the epidermal growth factor receptor/Raf/MEK/ERK pathway. DGKH is associated with attention deficit hyperactivity disorder, unipolar depression and schizophrenia (4).
References :
1. Rittiner, JE. et al: Overexpression of diacylglycerol kinase η enhances Gαq-coupled G protein-coupled receptor signaling. Mol Pharmacol. 2014; 85(5):800-810.
2. Ding, L. et al: The cloning and characterization of a novel human diacylglycerol kinase, DGKiota. J Biol Chem. 1998; 273(49):32746-52.
3. Buckland, AG. et al: Anionic phospholipids, interfacial binding and the regulation of cell functions. Biochim Biophys Acta. 2000; 1483(2):199-216.
4. Sakane, F. et al: Diacylglycerol kinases as emerging potential drug targets for a variety of diseases: An update. Front Cell Dev Biol. 2016; 4:82.
Product Sheets (By Lot #) :
Research Areas :Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Inflammation, PKA/PKC Pathway