DGKG, Active(D25-10H)
Description : Recombinant full-length human DGKG was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Species :Human
Tag :His tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :
Specific Activity : The specific activity of DGKG was determined to be 6.1 nmol/min/mg as per activity assay protocol
Purity :The purity of DGKG was determined to be >70% by densitometry, approx. MW 90kDa.
Storage, Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay
Molecular Weight :90 kDa
Gene Aliases : DGKgamma, DAGK3, DAGKgamma
Scientific Background :Diacylglycerol kinase gamma (DGKG) is a member of DGK type I subfamily which have EF-hand motifs and two cysteine-rich regions (C1 domain) in the regulatory domain. These enzymes catalyze the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins such as protein kinase C isoforms and RAS guanyl nucleotide-releasing proteins and some transient receptor potential channels. (1) DKAG has been associated with malignancies with its role as tumor suppressor in colorectal cancer. (2) DGKG is also implicated in regulation of cell cycle regulation. (3)
References :
- Sakane, F. et al. (1997) Diacylglycerol kinase J. Biol. Chem. 29, 1139-1143.
- Kai, M.et al (2017). Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer. Mol. Carcinog. 56, 1743-1752.
- Matsubara, T. et al. (2006). Nuclear transportation of diacylglycerol kinase γ and its possible function in the nucleus. J. Biol. Chem. 281, 6152-6164.
Product Sheets (By Lot #) :
Research Areas :Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Inflammation, PKA/PKC Pathway