ACKA Protein (A120-30H)
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Description :Full-length recombinant bacterial ACKA was expressed in E. coli cells using an N-terminal His tag.
Species :Bacteria
Tag :His tag
Expression System:E.coli
Sequence :Full length
Genbank Number :NP_416799
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :46 kDa
Gene Aliases :ECK2290; JW2293
Formulation :50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 2mM DTT, 25% glycerol
Scientific Background :ACKA belongs to the ASKHA superfamily of phosphor-transferases, and is the prototypic carboxylate kinase. Widely distributed in the bacteria and archaea domains. Catalyzes the phosphorylation of acetate.
References :
1. Buss KA, et al: (2001) Urkinase: structure of acetate kinase, a member of the ASKHA superfamily of phosphotransferases. J Bacteriol. 183(2):680-686.
2. http://www.uniprot.org/uniprot/F3MTX6
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