ROS1 (G2032R), Active(R14-12BG)
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Description :Recombinant human ROS1 (G2032R) (1881-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1881-end
Genbank Number :NM_002944
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~82 kDa
Gene Aliases :c-ros-1; MCF3; ROS
Scientific Background :ROS1 is a proto-oncogene and member of the sevenless subfamily of tyrosine kinase insulin receptor genes. ROS1 is highly-expressed in a variety of tumor cell lines and functions as a growth or differentiation factor receptor. The FIG gene can fuse with the ROS1 gene in glioblastoma cell lines (1). The resulting ROS1/FIG fusion protein is a constitutively activated tyrosine kinase. Direct interaction of ROS1 and the phosphatase SHP-1 can lead to efficient downregulation of ROS1-mediated signaling (2). Binding sites in the ROS1 cytoplasmic domain display high affinity binding to the SHP-1 N-terminal SH2 domain.
References :
1. Charest, A. et al: Fusion of FIG to the receptor tyrosine kinase ROS in a glioblastoma with an interstitial del(6)(q21q21). Genes Chromosomes Cancer 37: 58-71, 2003.
2. Biscup, C. et al: Visualization of SHP-1-target interaction. J Cell Sci. 2004 Oct 1;117(Pt 21):5165-78
Product Sheets (By Lot #) :
Research Areas :Receptor Tyrosine Kinases, Cancer