HDAC8, Active(H90-30G)
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Description :Full-length recombinant human HDAC8 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :BC050433
Specific Activity :Sample Enzyme Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :HDAC Assay, Western Blot
Molecular Weight :70 kDa
Gene Aliases :HDACL1; RPD3
Scientific Background :HDAC8 or Histone deacetylase 8 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. The protein encoded by this gene belongs to class I of the histone deacetylase family that catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors (1). There are 2 adjacent histidine residues in the predicted active site (His142 and His143) that are required for HDAC activity on H4 peptide substrates and core histones (2). The knockdown of HDAC8 by RNA interference inhibited the growth of human lung, colon, and cervical cancer cell lines.
References :
1. Buggy, J. J. et.al: Cloning and characterization of a novel human histone deacetylase, HDAC8. Biochem. J. 350: 199-226, 2000.
2. Vannini, A. et.al: Crystal structure of a eukaryotic zinc-dependent histone deacetylase, human HDAC8, complexed with a hydroxamic acid inhibitor. Proc. Nat. Acad. Sci. 101: 15064-15069, 2004.
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