RhoA Mutant (S188A) Protein (R44-32AH)
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Description :Recombinant human RhoA (S188A) (1-190) was expressed in E. coli cells using an N-terminal His tag.
Species :Human
Tag :His tag
Expression System:E.coli
Sequence :1-190
Genbank Number :NM_001664
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~23 kDa
Gene Aliases :ARH12; ARHA; RHO12; RHOH12
Scientific Background :RhoA is a member of the Rho gene family that interacts with protein kinases and serves as a target of activated GTPase. RhoA play an important role in the initial events of cell protrusion, whereas Rac1 and Cdc42 activate pathways implicated in reinforcement and stabilization of newly expanded protrusions. Rho mediates corneal epithelial migration in response to external stimuli by regulating the organization of the actin cytoskeleton (1). The inhibition of RhoA signaling is required for both vaccinia morphogenesis and virus-induced cell motility. Vaccinia inhibits RhoA signalling using the viral protein F11 and this can enhance the spread of infection (2). It has been reported that cGMP/cGMP-dependent protein kinase can phosphorylate RhoA at Ser188 in vitro (3).
References :
1. Wu, K. Y.et.al: Local translation of RhoA regulates growth cone collapse. Nature 436: 1020-1024, 2005.
2.Valderrama, F.et.al: Vaccinia virus-induced cell motility requires F11L-mediated inhibition of RhoA signaling. Science 311: 377-381, 2006.
3. Sawada N. et.al: cGMP-dependent protein kinase phosphorylates and inactivates RhoA. Biochem. Biophys. Res. Commun. 280:798-805(2001).
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Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology