PsmCas13b (CS13B-E312H)

PsmCas13b (CS13B-E312H)

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Description: Recombinant full-length Prevotella sp. MA2016 Cas13b (PsmCas13b) was expressed in E. coli with a N-terminal His tag.

Species: Alicyclobacillus acidiphilus

Tag: Tag free

Expression System: E.Coli cells

Genbank Number: WP_028912271.

Purity: Sample purity data. For lot-specific data, please refer to the specific datasheet.



Activity:  Sample activity data. For lot-specific data, please refer to the specific datasheet.

 

Storage and Stability:  Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight: 128 kDa

Gene Aliases : c2c1, CRISPR-associated endonuclease C2c1,

Scientific Background: CRISPR-Cas systems constitute the adaptive immune system in bacteria via RNA-guided endonucleases (1,2). This system has been redesigned to create an exemplary genome editing tool for application to RNA-based therapeutics development (3). PsmCas13b (Prevotella sp. MA2016 Cas13b) is a CRISPR-Cas effector that belongs to the class 2 subtype VI-B, and has been shown to effectively target and silence distinct mammalian ssRNA viruses (3,4). Cas13b processes its own CRISPR array with short and long direct repeats, cleaves target RNA, and exhibits collateral RNase activity (4).

References : 

  1. Horvath, P., et al. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
  2. Morange, M. What history tells us XXXVII.  CRISPR-Cas: The disconvery of an immune system in prokaryotes.  Biosci. 40(2):221-223.
  3. Smargon, A. A., et al. Cas13b is a Type VI-B CRISPR-associated RNA-Guided RNAse differentially regulated by accessory proteins Csx27 and Csx28. Mol. Cell. 65(4):618-630.
  4. Freije, C. A., et al. Programmable Inhibition and Detection of RNA Viruses Using Cas13. Mol. Cell. 76(5):826-837.


Product Sheets (By Lot #) :

T4334-1