Cas12 (LbCpf1), Active (C12CR-E241H)

Cas12 (LbCpf1), Active (C12CR-E241H)

  • $190.00


Description :Recombinant Lachnospiraceae Cas12 (LbCpf1) (1-1227) was expressed in E. coli with a C-terminal His tag.

Species :Lachnospiraceae

Tag : His tag

Expression System:E.coli

Sequence :1-1227

Genbank Number :ATB19154

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Activity: Sample Purity Data. For specific information on a given lot, see the related technical datasheet.

Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight : aprrox130 kDa (calculated MW approx 140 kDa)

Gene Aliases :Cpf1 (synthetic construct)

Scientific Background :The severe acute respiratory syndrome related novel coronavirus SARS-CoV-2 has caused the pandemic of the respiratory diseases (COVID-19) around the world in 2020 (1). Developing rapid and sensitive diagnostic tools is key for successful patient management and curbing the disease spread. Several CRISPR-Cas12 based SARS-CoV-2 detection assays have been developed (2,3). Comparing to conventional RT-PCR based tests that are hindered by the need for expensive lab instrumentation, Cas12 based tests are ultrasensitive, rapid and in low-cost POCT formats.

References :
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Broughton JP, et al: Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR-based DETECTR lateral flow assay. medRxiv doi: 2020.03.06.20032334.

3. Lucia C, et al: An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on CRISPR-Cas12. bioRxiv. doi: 2020.02.29.971127.

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