AsCas12a, Active (CS12A-E311H)

AsCas12a, Active (CS12A-E311H)

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Description: Recombinant Acidaminococcus AsCas12a was expressed in E. coli with a C-terminal His tag.

Species: Acidaminococcus

Tag:  HIS tag

Expression System: E.Coli cells

Genbank Number: WP_021736722

Purity: Sample purity data. For lot-specific data, please refer to the specific datasheet.



Activity:  Sample activity data. For lot-specific data, please refer to the specific datasheet.

Storage and Stability:  Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight: 130 kDa

Gene Aliases : Cpf1, AsCpf1, CRISPR-associated endonuclease Cpf1

Scientific Background: CRISPR (clustered regularly interspaced short palindromic repeat) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been redesigned to create an exemplary genome editing tool for application to RNA-based therapeutics development (3). AsCas12a is a CRISPR-associated endonuclease from Acidaminococcus and it can be engineered to achieve combinatorial genetic screening with high efficiency (2). Engineered AsCas12a further enables accurate editing in hematopoietic stem cells, induced pluripotent stem (iPS) cells, and natural killer (NK) cells and helps fight against sickle cell disease (4).

References : 

  1. Horvath, P., et al. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
  2. Morange, M. What history tells us XXXVII.  CRISPR-Cas: The disconvery of an immune system in prokaryotes.  Biosci. 40(2):221-223.
  3. Gier, R.A., et al. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 2020, 11(1):1-9.
  4. DeLong, R. Ushering in a new era of RNA-based therapies. Commun Biol. doi: https://doi.org/10.1038/s42003-021-02150-w


Product Sheets (By Lot #) :

O3970-1