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Anti-2019-nCoV N-Protein (C19NP-60H)

Anti-2019-nCoV N-Protein (C19NP-60H)

  • $165.00


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Description :Recombinant Human Monoclonal Antibody

Specificity :Recognizes the 2019-nCoV N-Protein

Cited Applications :ELISA, Western Blot
Ideal working dilutions for each application should be empirically determined by the investigator.

Cross Reactivity :SARS-CoV N-Protein

Host / Isotype / Clone# :Human IgG

Stability :1yr at –20oC from date of shipment.

Scientific Background :CoV N-Protein / Nucleocapsid protein is an RNA-binding protein primarily involved in binding to the viral RNA genome during packaging of viral RNA into a helical ribonucleoproteins or nucelocapsid structure (1). In addition, N-protein regulates viral RNA synthesis during replication/transcription and modulates host-pathogen interactions, such as actin reorganization, host cell cycle progression, and apoptosis (2). N-Protein is abundantly expressed during infection and antibodies against N-Protein have been detected in confirmed cases of Covid-19. Therefore, N-Protein is a potent antigen that provides basis for future vaccine and diagnostic kit development (3).

References:
1. McBride R, et al: The coronavirus nucleocapsid is a multifunctional protein. Viruses. 2014, 6:2991-3018.

2. Surjit M, et al: The nucleocapsid protein of severe acute respiratory syndrome-coronavirus inhibits the activity of cyclin-cyclin-dependent kinase complex and blocks S phase progression in mammalian cells. J Biol Chem. 2006, 281:10669-10681.

3. Zeng W, et al: Biochemical characterization of SARS-CoV-2 nucleocapsid protein. Biochem Biophys Res Commun. 2020, 527(3):618‐623.

Assay Data :Binding of Anti-2019-nCoV N-Protein Antibody (C19NP-60H) to immobilized 2019-nCoV N-Protein (C19NP-G242H) determined by ELISA using Goat anti-human Fc.

Western Blot :Representative western blot of Anti-2019-nCoV N-Protein Antibody (C19NP-60H) (1:1000) using 1 µg of 2019-nCoV N-protein (C19NP-G242H).

Product Sheets (By Lot #) :

S3367-9.pdf

S3655-20.pdf