2019-nCoV Spike protein S1 (T19R, G142D, del157-158, L452R, T478K, D614G, P681R) C19S1-G231LH

2019-nCoV Spike protein S1 (T19R, G142D, del157-158, L452R, T478K, D614G, P681R) C19S1-G231LH

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Description :   Recombinant 2019-nCoV Spike protein S1 (T19R, G142D, ∆157-158, L452R, T478K, D614G, P681R) (16-685) was expressed in CHO cells using a C-terminal His- tag.

Species : Human

Tag :HIS tag

Expression System: CHO cells

Sequence : Full length

Genbank Number : MN908947

Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight : 130 kDa

Gene Aliases : 2019-nCoV s1, SARS-CoV-2 spike S1, SARS-CoV-2 S1, novel coronavirus spike s1, nCoV spike s1, coronavirus spike S1.

Scientific Background : Since 2020, the novel coronavirus SARS-CoV-2 has caused respiratory disease (COVID-19) pandemic around the world. The coronavirus spike glycoprotein (S) is a type I transmembrane protein that contains the S1 and S2 subunits and it interacts with the host cell angiotensin-converting enzyme 2 (ACE2) to facilitate viral genome entry (1). The delta variant (B.1.617.2 lineage), first discovered in India, possesses several key mutations that may explain its replacement over the alpha variant. The receptor binding domain (RBD) mutations L452R and T478K lead to increased binding to ACE2 and decreased neutralization potential (2). Mutation D614G increases viral infectivity; mutation P681R in the furin cleavage site promotes the cleavage rate of the S1-S2 making the virus more transmissible and more infectious. As new variants displace the first-wave virus, it is pivotal to evaluate their transmissibility, virulence, and their possible tendency to escape antibody neutralization (3).

References :

  1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.
  2. Planas D, et al: Reduced sensitivity of SARS-CoV-2 variant Delta to antibody neutralization. Nature. 2021, 596:276-280.
  3. Cherian S, et al: SARS-CoV-2 spike mutations, L452R, T478K, E484Q and P681R, in the second wave of COVID-19 in Maharashtra, India. Microorganisms. 2021, 9, 1542.

 

Product Sheets (By Lot #) :  O3838-16